Yes.
As a test, I just created a project template containing
An audio track with Channel Tools in the FX bin and an audio clip in the track
A MIDI track with the Event Filter in the FX bin
An Instrument track with DPro loaded
A Master bus with FabFilter Pro-Q
Metronome and Preview Buses
And a Reverb Bus with Sonitus Reverb
Created a new project with the template. When loading, the project wanted the path to the audio clip. Other than that, the template loaded without incident.
It may be the plug-ins selected, it may be the routing used. Difficult to say without more details about the template. If, I have the plug-ins used in the template, I would be glad to load and test it for you.
While a template should work, another solution is to keep the file as an empty project and copy it whenever you need the layout.