Gain knob is not required in Studio One. What you can do in Studio One is simply edit the volume of any audio event on a track
(individually which you cannot do with the gain knob effecting a whole track so its actually better) by simply grabbing the gain handle and raising or lowering. Unlike Sonar you can see the changes in waveform height immediately. After a while you can get pretty savvy at just looking at waveforms and getting all their rms levels very consistent. Before you start a mix.
If you track properly as well using a VU meter you will also never need a gain knob either. It is when you get material from someone else that has done the tracking that you are more likely to see inconsistent rms levels on tracks. In that situation I tend to open them all up one by one in an editor and with a VU over the tracks make all the adjustments there. It is far more accurate than fiddling a gain knob.
As far as the mixer is concerned it depends on who you are talking to. I don't like the Sonar mixer at all but love the Studio One mixer. So sorry it is not the best mixer in the world! Mixbus mixer is also pretty cool too.