Assuming you have enough audio outputs to support the Sub on an output by itself than yes you can do this fairly easily.
Just create 2 additional busses on your project and add a send for one of the new busses in your existing Main output bus. Then direct your main output to the other new buss.
Add eq to each and create the crossover curve you want to use. For instance you could set a hipass on one and direct that to your main speakers and a lowpass on the other and direct to your Subwoofer.
This is off the top of my head and is over simplified but should get you on the right track.