SonicExplorer
As for a difference in audio quality, I could have sworn when I ran preliminary A/B tests when S5 was first released that I could hear more clarity and smoothness. Just an overall better sounding result with the 64-bit mix engine. I want to say the result was smoother and punchier, just overall higher-end professional sounding. It was subtle but if you are a picky person it was definitely noticeable. But I simply can't recall back that far to be honest.
If you have a single track that started in the analog domain (including sample libraries!), it's inherent noise will absolutely bury the level at which 32 bit calculation errors start to appear.
Since in this case the errors do not accumulate over time and do not accumulate rapidly, there are simply not enough calculations done on a single track in the mix engine* to raise the error level enough to be anywhere close to the noise already in the tracks, much less audible.
And obviously adding multiple tracks like this together will not make calculation errors add faster than the noise from the individual tracks.
In fact most of the 32 bit calculation errors won't even make it into 24 bit audio output, much less be above the noise floor due to other causes, much less be audible.
You will find people who believe they hear a difference, but inevitably they are not using a reliable method of comparison and what they claim to hear and the circumstances where they claim to hear it simply do not match what the calculation errors would sound like if they were audible.
* there are circumstances where 64 bit calculations are necessary due to the extreme number of calculations done, but this is not one of them.