I don't imagine a power transformer can make much difference in tone but a good output transformer can. Otherwise Mercury Magnetics would likely go out of business, why pay more for the same thing? The first preamp tube can make a huge difference, sometimes the second... but speakers make the biggest difference.
Having said all that, I can take pretty much any amplifier and dial in "my tone". Like Clapton on the Cream reunion tour, using a Stratocaster and what looked like a narrow panel tweed Fender amp, and a Leslie (he used a Leslie on Derek and the Dominoes in his chain too)... but Crossroads.. sounded like well, Cream.
That doesn't stop me from wanting more amps and OD pedals.
I do tend to change speakers in my favorite amps and use NOS preamp tubes in V1.
I guess what I mean to say is your settings make the most difference in your tone. I sound like me no matter what I play through. Eh...
The pickups you put in a guitar make more difference, to my ears than transformers or tubes... maybe more than speakers. Huge difference between a PAF and a JB. Fast Tracks and CS 54s.
edit> I almost forgot. Even knowing all this... I am thinking of selling some stuff to get a Dr. Z Antidote head. It sound's like the ultimate 45w Marshall style head to me, at least for the money. Used ones on Reverb.com for around 1599.00 new 1999.00. You only live once, may as well live well, huh? Check out the Cast-engineering Texas Flood pedal. TONE.
J