If you are heading into the land of heavy duty sample streaming: VSL, EW HS, HB, etc., another drive would be helpful.
Even with standard 7200 rpm drives, performance can be quite decent, though load times, and general polyphony counts can benefit greatly from SSD's.
If you intend to get many libraries, spreading them out over multiple hard drives also helps greatly.
In general, if using just one computer: one drive for o/s and programs; one drive for audio files; one drive for samples (two when the number of sample libraries starts to seriously increase).
Finally, putting samples on the o/s drive is kind of a last resort, as streaming from the o/s drive is generally problematic, as that drive has to do many other things.