The occasional off beat hit? In my experience Tambourine players sometimes lose focus on the tambourine during the song and push and pull on the timing in a way that is more than swing. dropping a beat in a measure or adding one. Not all the time, but sometimes.
And I'm not trying to say that it's just bad musicians who play tambourine. It's often singers who have a lot of other things to think about.
The other thing I find is with acoustic groups that tend to pull out tambourines, the tambourine goes into lots of microphones on the stage sometimes causing interesting phasing fx. So for this reason, copying the track and nudging it a few milliseconds, then using a ctrl+drag sign line at a very low volume and panning left and right might be interesting.