The normal alphabet of hard drives is:
A:/B: for floppies
C:OS
D: recovery (if that is installed as w/ many OEM OS's - leave that be if you want to be able to restore your OS)
E: CD/DVD drive - which can be changed if you want - Windows will recognise it no matter what the name.
It is best to develop a system so if you buy a new computer you can just slide your data drive(s) in and SOnar etc. will find the files without repointing.
As John sez, make sure your power supply can handle the extra drives. Most can, but ....