Ya, I new what you meant as that's what they are called on a mixer.
Ok Same difference, but make sure you follow each signal chain and that all the busses are therefore assigned correctly to the master.
It can only be a signal path is not complete or you have something muted. Try routing the offending "Channel" to the master and see what happens. Remove or bypass all efxs too.
And I don't understand what you mean by a folder? There are folders for midi soft synths but not for audio, Audio is in a audio track. I'm not sure what happens if you drop an audio clip into a synth folder???
I would never use layers anyways, much easier to just record a new track for each take and work with those. Layers seem to cause a lot of people problems and I can see why.
You can always insert a few new audio tracks and drag those layers to them too. Just be careful with draging out of sync.