There is a sub forum for the vs 100 here where you might find some good reading.
One thing to check is that your tracks outputs are sent to either a master bus or directly to the VS100 outputs.
If your using a Master bus make sure it's outputs are set to the VS100.
Every track has a input and output.
Every soft synth has an output
Every EFX send and Bus has an output.
These all have to be checked and set correctly.
Make sure the VS100 output you choose is the one that your talking about (with the RCA jacks). There may be more than one output option.
Often I have lost output when switching audio interfaces and they default to SPDIF output. My guess is it is still confused about the audigy.