tomixornot
Line/instrument input are basically the same - for keyboard/synthesizer with 1/4 inch jack output, either stereo (using 2 inputs pan left/right) or mono. You can also plug in the CD player output, via RCA plugs (red/white) to 1/4 inch cable/converter to line input.
Some line/instrument input also has a button to engage the 'hi-z' - this is for guitar/bass to plug in directly.
Without the 'hi-z' button, you probably need to plug the guitar to a direct-box, and the direct-box output to line/instrument input.
At times, without a direct-box, I do plug in my guitar to my mixer's line/mic input (accepts both XLR and 1/4 inch plug) and use the mic gain to boost the signal.. but I'm probably doing it the wrong way.
Sorry, this is mostly wrong. There is a big difference between instrument and line inputs.
A line input is designed to accept a "hi power" signal from a piece of powered equipment, CD player, synth, etc. Anything that has it's own power source and provides an "amplified" signal (at line level) as compaired to a microphone output.
An instrument input is high impedence (Hi Z) and is designed for passive guitar pickups and other similar transducers that produce voltage levels of the same order as line level, but at very low power. The low impedence (Low Z) of a line input would drag down the voltage produced by a guitar pickup, making the signal quiet and also affecting the "tone" by interacting with the tone capacitors in the guitar.
A passive DI box is basicaly an audio grade transformer that provides a Hi Z input for your guitar and a MIC level output. An active DI box contains an amplifier and can provide a true line level output.
The inputs on some audio interfaces are often marked as Line/Instr. In this case there should be a button to switch between the two, often marked Hi Z. If you plug your guitar directly into the interface you need to select the Instr/Hi Z option.
If you use a seperate DI box you use the mic or line input, not the instr input.